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互养菌（Syntrophus） 1

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The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

《环境科学与工程前沿（英文）》 2018年 第12卷 第1期 doi: 10.1007/s11783-018-1016-0

摘要： The feasibility of using -JS45 system in removing nitrobenzene from sediments was conducted. However, it was observed that nitrobenzene degraded rapidly and was removed completely within 20 days in native sediments, raising the possibility that indigenous microorganisms may play important roles in nitrobenzene degradation. Consequently, this study aimed to verify this possibility and investigate the potential nitrobenzene degraders among indigenous microorganisms in sediments. The abundance of inoculated strain JS45 and indigenous bacteria in sediments was quantified using real-time polymerase chain reaction. Furthermore, community structure of the indigenous bacteria was analyzed through high throughput sequencing based on Illumina MiSeq platform. The results showed that indigenous bacteria in native sediments were abundant, approximately 10 CFU/g dry weight, which is about six orders of magnitude higher than that in fertile soils. In addition, the levels of indigenous ( , , , and ) and ( , , , , and ) increased significantly during nitrobenzene removal. Their quantities sharply decreased after nitrobenzene was removed completely, except for and . Based on the results, it can be concluded that indigenous microorganisms including and can have great potential for removing nitrobenzene from sediments. Although . - JS45 system was set up in an attempt to eliminate nitrobenzene from sediments, and the system did not meet the expectation. The findings still provide valuable information on enhancing nitrobenzene removal by optimizing the sediment conditions for better growth of indigenous and .

关键词： Community structure     Indigenous microorganisms     Nitrobenzene     Plant-microbe associated remediation     Sediment    

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Response of soil microorganisms to vegetational succession in Ziwuling Forest

Hong ZHANG, Jialong LU, Shiwei ZHAO,

《环境科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 421-427 doi: 10.1007/s11783-009-0147-8

摘要： This study investigates the changes in soil microbial populations as vegetational succession progress from abandoned farmland to climax forest in the Ziwuling Forest, which is located in the northern part of the Loess Plateau, China. Different organic C and total extractable N between the fumigated and non-fumigated soils were assumed to be released from soil microorganisms. Soil microbial C was calculated using ec=0.38 and microbial N was calculated using en=0.45. The released P was converted to microbial biomass P using ep=0.40. Soil bacteria, fungi, and actinomycetes increased as the vegetational succession progressed. Microbial C was of the highest amount in farmland. Microbial C, N, and P generally increased from abandoned land to climax community. The results indicated significant (<0.05) diversities of soil microbial biomass under different vegetation. There was a significant correlation between microbial biomass and soil nutrients. Knowledge about soil microbial populations is important for forecasting vegetational succession and determining the ecological condition of the environment.

关键词： soil microorganisms     vegetational succession     Ziwuling Forest    

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Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第6期   页码 844-850 doi: 10.1007/s11783-013-0487-2

摘要： In this study, microorganisms (named B111) were immobilized on polyvinyl alcohol microspheres prepared by the inverse suspension crosslinked method. The biodegradation of bisphenol A (BPA) and 4-hydroxybenzaldehyde, a degradation product of BPA, by free and immobilized B111 was investigated. The BPA degradation studies were carried out at initial BPA concentrations ranging from 25 to 150 mg·L . The affinity constant K and maximum degradation rate were 98.3 mg·L and 19.7 mg·mg VSS·d for free B111, as well as 87.2 mg·L and 21.1 mg·mg VSS·d for immobilized B111, respectively. 16S rDNA gene sequence analyses confirmed that the dominant genera were and for BPA biodegradation in microorganisms B111.

关键词： bisphenol A     polyvinyl alcohol     immobilized microorganisms     4-hydroxybenzaldehyde    

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Advances in airborne microorganisms detection using biosensors: A critical review

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-021-1420-8

摘要：

Humanity has been facing the threat of a variety of infectious diseases. Airborne microorganisms can cause airborne infectious diseases, which spread rapidly and extensively, causing huge losses to human society on a global scale. In recent years, the detection technology for airborne microorganisms has developed rapidly; it can be roughly divided into biochemical, immune, and molecular technologies.

关键词： Biosensor     Airborne microorganisms     Microbiological detection technology    

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Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1504-5

摘要：

• Diversity and detection methods of pathogenic microorganisms in sludge.

关键词： Sludge treatment     Pathogenic microorganisms     Inactivation mechanisms     Exposure risks     Land application    

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Firm innovation system: Paths for enhancing corporate indigenous innovation capability

Jin CHEN, Ximing YIN, Jizhen LI

《工程管理前沿（英文）》 2020年 第7卷 第3期   页码 404-412 doi: 10.1007/s42524-020-0116-2

摘要： To obtain a sustainable competitive advantage and achieve global innovation leadership, firms in China and other emerging economies must develop strong indigenous innovation capabilities through the coevolution of strategy, organization, resources, and culture. Drawing on current innovation management theories and practices, this study proposes four systematic paths for improving firm innovation systems (FISs), namely, the R&D-based internal collaborative FIS, the strategic vision-driven FIS, the open and user-driven FIS, and the holistic ecosystem-driven FIS. This study contributes to the systematic approach for enhancing corporate indigenous innovation capability based on FISs. Moreover, this study provides theoretical and practical insights for China as well as other developing countries to cultivate world-class enterprises and build an innovative nation.

关键词： innovation-driven strategy     firm innovation system     national innovation system     holistic innovation theory     core competence    

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Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

《环境科学与工程前沿（英文）》 2022年 第16卷 第5期 doi: 10.1007/s11783-022-1567-y

摘要：

● Evaluated three methods for determining the consortia’s growth kinetics.

关键词： Biodegradation     1     4-Dioxane     Kinetics     Microbial consortium     16S rRNA    

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Genetic diversity and population structure of indigenous chicken breeds in South China

Xunhe HUANG,Jinfeng ZHANG,Danlin HE,Xiquan ZHANG,Fusheng ZHONG,Weina LI,Qingmei ZHENG,Jiebo CHEN,Bingwang DU

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 97-101 doi: 10.15302/J-FASE-2016102

摘要： A total of 587 individuals from 12 indigenous chicken breeds from South China and two commercial breeds were genotyped for 26 microsatellites to investigate the genetic diversity and population structure. All microsatellites were found to be polymorphic. The number of alleles per locus ranged from 5 to 36, with an average of 12.10 ± 7.00 (SE). All breeds, except White Recessive Rock, had high allelic polymorphism (>0.5). Higher genetic diversity was revealed in the indigenous chicken breeds rather than in the commercial breeds. Potential introgression from the commercial breeds into the indigenous chickens was also detected. The population structure of these indigenous chicken breeds could be explained by their geographical distribution, which suggested the presence of independent history of breed formation. Data generated in this study will provide valuable information to the conservation for indigenous chicken breeds in future.

关键词： microsatellites     genetic diversity     population structure     indigenous chicken     South China     conservation    

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Diversity and distribution of proteorhodopsin-containing microorganisms in marine environments

Bo WEI

《环境科学与工程前沿（英文）》 2012年 第6卷 第1期   页码 98-106 doi: 10.1007/s11783-010-0278-y

摘要： Proteorhodopsin (PR) is a recently discovered protein involved in the utilization of light energy. Several studies have shown that PR-containing microorganisms are widespread and compose a large proportion of the biomass in marine ecosystems. A better understanding of the ecological role of PR will help clarify the effect of the global flow of energy and the carbon cycle on marine communities. In this study, a bioinformatical database of PR codon sequences, the Global Distribution Database of Proteorhodopsin (GDDP), as a tool for analyzing the diversity and distribution of PR-containing microorganisms in marine environments throughout the world was designed. The community structure of PR microorganisms were also compared using PCR assays and UniFrac analyses of 12 samples collected from three water layers (0, 75, and 200 m) at four representative sites in the Pacific, Atlantic, and Indian Oceans. The results indicate that PR-containing microorganisms can be grouped into two distribution types: widespread and location-specific. Representative cases of the former include SAR11-PR and HOT2C01-PR. Interestingly, PR communities cluster by geographic locale but not by water depth.

关键词： proteorhodopsin (PR)     microorganism community     diversity     marine environments    

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Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1426-2

摘要：

• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.

关键词： Ammonia oxidizing archaea (AOA)     Complete ammonia oxidizing (comammox) bacteria     Dissimilatory nitrate reduction to ammonium (DNRA) bacteria     Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms     Engineering application    

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Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

《环境科学与工程前沿（英文）》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1057-4

摘要：

Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium than chromium.

Indigenous Cd-tolerant EAB cytoplasm located more chromium than cadmium.

Simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB was achieved.

Current accelerated the harboring of cadmium in EAB at an initial 2 h.

Current directed the accumulation of more chromium in EAB over time.

关键词： Microbial electrolysis cell     Electrochemically active bacteria     Cd-tolerant bacteria     Cd(II) and Cr(VI)     Fluorescence probe    

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Technologies for extracting lipids from oleaginous microorganisms for biodiesel production

Cunwen WANG, Lu CHEN, Bajpai RAKESH, Yuanhang QIN, Renliang LV

《能源前沿（英文）》 2012年 第6卷 第3期   页码 266-274 doi: 10.1007/s11708-012-0193-y

摘要： Recently, biodiesel has received much more attention. Soybean oil, rapeseed oil, palm oil and corn oil are primary feedstock for biodiesel production. However, biodiesel production from these traditional oil-rich crops is limited by land availability, climate, and environmental and social issues regarding the use of feed and food crops for fuel. Oleaginous microorganisms, including microalgae, bacteria, yeast and fungi can be cultivated with high lipid contents and used as promising feedstock for biodiesel production. However, the high cost of biodiesel production using oil microorganisms has been the biggest obstacle for its industrialization. The process of biodiesel production from microorganisms involves many steps, of which the lipids extraction is the most important and costly. Therefore, searching for an effective and economical extraction system is critical. Various approaches of lipids extraction are discussed in this review, including traditional extraction procedures such as solvent extraction, pressing and solvent integrated extraction, as well as some new procedures.

关键词： oleaginous microorganisms     biodiesel     solvent extraction     ultrasound-assisted extraction     microwave-assisted extraction     supercritical fluid extraction (SFE)    

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Unveiling the interaction mechanisms of key functional microorganisms in the partial denitrification-anammox

《环境科学与工程前沿（英文）》 2023年 第17卷 第8期 doi: 10.1007/s11783-023-1703-3

摘要：

● The availability of PD-anammox was investigated with higher NO3––N concentration.

关键词： PD-anammox process     Nitrite accumulation     COD     Microbial interaction    

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Bioleaching of spent Ni-Cd batteries and phylogenetic analysis of an acidophilic strain in acidified sludge

ZHAO Ling, WANG Liang, YANG Dong, ZHU Nanwen

《环境科学与工程前沿（英文）》 2007年 第1卷 第4期   页码 459-465 doi: 10.1007/s11783-007-0073-6

摘要： Biohydrometallurgy is a novel method to recycle discarded batteries, in which sewage sludge is used as microorganisms and culture due to the presence of indigenous . A two-step continuous flow leaching system co

关键词： Biohydrometallurgy     discarded     indigenous     two-step continuous     presence    

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Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

《环境科学与工程前沿（英文）》 2018年 第12卷 第2期 doi: 10.1007/s11783-017-0986-7

摘要： Transport of engineered antibiotic resistance plasmids in porous media has been reported to potentially cause significant spreading of antibiotic resistance in the environment. In this work, transport of an indigenous resistance plasmid pK5 in porous media was investigated through packed column experiments. At identical ionic strengths in CaCl solutions, the breakthroughs of pK5 from soil columns were very close to those from quartz sand columns, indicating that transport of pK5 in quartz sand and soil was similar. A similarity in transport behavior was also found between pK5 and an engineered plasmid pBR322 that has approximately the same number of base pairs as pK5. The influence of surfactants, a major group of constituents in soil solutions, was examined using an engineered plasmid pcDNA3.1(+)/myc-His A. The impact of an anionic surfactant, sodium dodecyl sulfate (SDS), was negligible at concentrations up to 200 mg·L . Cetyltrimethyl ammonium bromide (CTAB), a cationic surfactant, was found to significantly enhance plasmid adsorption at high concentrations. However, at environmentally relevant concentrations (<1 mg·L ), the effect of this surfactant was also minimal. The negligible impact of surfactants and the similarity between the transport of engineered and indigenous plasmids indicate that under environmentally relevant conditions, indigenous plasmids in soil also have the potential to transport over long distances and lead to the spreading of antibiotic resistance.

关键词： Indigenous plasmid     Transport     Porous media     Surfactants    

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标题 作者 时间 类型 操作

The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

期刊论文

Response of soil microorganisms to vegetational succession in Ziwuling Forest

Hong ZHANG, Jialong LU, Shiwei ZHAO,

期刊论文

Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

期刊论文

Advances in airborne microorganisms detection using biosensors: A critical review

期刊论文

Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

期刊论文

Firm innovation system: Paths for enhancing corporate indigenous innovation capability

Jin CHEN, Ximing YIN, Jizhen LI

期刊论文

Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

期刊论文

Genetic diversity and population structure of indigenous chicken breeds in South China

Xunhe HUANG,Jinfeng ZHANG,Danlin HE,Xiquan ZHANG,Fusheng ZHONG,Weina LI,Qingmei ZHENG,Jiebo CHEN,Bingwang DU

期刊论文

Diversity and distribution of proteorhodopsin-containing microorganisms in marine environments

Bo WEI

期刊论文

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

期刊论文

Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

期刊论文

Technologies for extracting lipids from oleaginous microorganisms for biodiesel production

Cunwen WANG, Lu CHEN, Bajpai RAKESH, Yuanhang QIN, Renliang LV

期刊论文

Unveiling the interaction mechanisms of key functional microorganisms in the partial denitrification-anammox

期刊论文

Bioleaching of spent Ni-Cd batteries and phylogenetic analysis of an acidophilic strain in acidified sludge

ZHAO Ling, WANG Liang, YANG Dong, ZHU Nanwen

期刊论文

Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

期刊论文